ELF-EMFs
Induced Effects on Cell Lines: Controlling ELF Generation in Laboratory
Marco Farina
,
Marcello Farina
,
Maria A. Mariggio
,
Tiziana Pietrangelo
,
Joseph J. Stupak
,
Antonio Morini
and
Giorgio Fano
The aim of this paper is to discuss the effects of the exposure to Extremely Low Frequency ElectroMagnetic Fields (ELF-EMFs) on non- and excitable cells using in vitro cell models, namely neuron-like cell line (PC12), glioblastoma GL15 as glial model and C2C12 myocytes as muscle model, focusing our attention on standardized protocols for ELF-EMFs generation and exposure. A major issue in laboratory -and likely in natura- studies about possible biological effects of ELF waves is the difficulty in providing standard, reproducible environmental conditions. Hence, as part of the work we have developed an exposure system including a probing scanner, able to sample a given volume and to measure the time-varying magnetic field vector. The system allows detection, monitoring and removal of electromagnetic noise sources, as well as means to assess field homogeneity in terms of intensity and polarization.