volume | PIER Journals

Abstract

In this study, we introduce a dual-modal fluorescence hyperspectral micro-CT system developed for e.g. bioimaging applications. The system integrates an X-ray computed tomography (CT) module with a custom-designed hyperspectral fluorescence imaging module, achieving high-resolution structural imaging with detailed molecular-level insights. With a spectral resolution of 10 nm across the wavelength range of 450–750 nm, the hyperspectral fluorescence imaging module enables a fine compositional analysis. Using surface-modified nanoparticles, we demonstrate the system's capability to capture fluorescence under both X-ray and UV excitation. Imaging experiments on a mouse model further highlight the system's ability to generate comprehensive Four-dimensional (4D) datasets that integrate spatial, spectral, and structural information. To the best of our knowledge, no such a dual-modal system or the like has even been reported before. This dual-modal approach enhances the visualization and analysis of biological tissues, offering promising applications in e.g. disease diagnosis, surgical guidance, and preclinical research.

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Dr. Jing Luo

Ms. He Zhu

Dr. Raheel Ahmed Janjua

Dr. Wenbin Ji

Dr. Ruili Zhang

Dr. Junbo Liang

Prof. Sailing He