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PIER PIER B PIER C PIER M PIER Letters
2020-11-25
Multi-Laser Scanning Confocal Fluorescent Endoscopy Scheme for Subcellular Imaging (Invited)
By
Xiaomin Zheng,

    Dr. Xiaomin Zheng

    College of Physics and Optoelectronics Engineering

    Shenzhen University

    China

    Homepage

Xiang Li,

    Dr. Xiang Li

    Shenzhen University

    China

    Homepage

Qiao Lin,

    Dr. Qiao Lin

    China Pharmaceutical University

    China

    Homepage

Jiajie Chen,

    Professor Jiajie Chen

    Shenzhen University

    China

    Homepage

Yueqing Gu,

    Dr. Yueqing Gu

    China Pharmaceutical University

    China

    Homepage

Yonghong Shao

    Dr. Yonghong Shao

    Shenzhen University

    China

    Homepage

Progress In Electromagnetics Research, Vol. 169, 17-23, 2020
doi:10.2528/PIER20092201
Abstract
Fluorescence confocal laser scanning endomicroscopy is a novel tool combining confocal microscopy and endoscopy for in-vivo subcellular structure imaging with comparable resolution as the traditional microscope. In this paper, we propose a three-channel fluorescence confocal microscopy system based on fiber bundle and two excitation laser lines of 488nm and 650nm. Three fluorescent photomultiplier detecting channels of red, green and blue can record multi-color fluorescence signals from single sample site simultaneously. And its ability for in-vivo multi-channel fluorescence detection at subcellular level is verified. Moreover, the system has achieved an effective field of view of 154μm in diameter with high resolution. With its multi-laser scanning, multi-channel detection, flexible probing, and in-vivo imaging abilities it will become a powerful tool in bio-chemical research and diagnostics, such as the investigation of the transport mechanism of nano-drugs in small animals.
Citation
Xiaomin Zheng, Xiang Li, Qiao Lin, Jiajie Chen, Yueqing Gu, and Yonghong Shao, "Multi-Laser Scanning Confocal Fluorescent Endoscopy Scheme for Subcellular Imaging (Invited)," Progress In Electromagnetics Research, Vol. 169, 17-23, 2020.
doi:10.2528/PIER20092201
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