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PIER PIER B PIER C PIER M PIER Letters
2018-03-05
Exploratory Study on Light-Sheet Based Three-Dimensional Surface Topography
By
Fuhong Cai,

    Dr. Fuhong Cai

    Mechanical and Electrical Engineering College

    Hainan University

    China

    Homepage

Jie Chen,

    Dr. Jie Chen

    College of Mechanical and Electrical Engineering

    Hainan University

    China

    Homepage

Chunling Zhou,

    Dr. Chunling Zhou

    Zhejiang University

    China

    Homepage

Xuan Zhu,

    Dr. Xuan Zhu

    College of Mechanical and Electrical Engineering

    Hainan University

    China

    Homepage

Sailing He

    Prof. Sailing He

    Department of Electromagnetic Theory

    Royal Institute of Technology

    Sweden

    Homepage

Progress In Electromagnetics Research, Vol. 161, 11-18, 2018
doi:10.2528/PIER18012703
Abstract
Light-sheet microscopy has attracted considerable attention since it is a fluorescence imaging technique with rapid optical sectioning capabilities for transparent sample. In our study, we report a new application based on light-sheet microscopy for exploratory investigating three-dimensional surface topography of opaque sample. Instead of using inelastic scattering fluorescent signal, our method utilizes the elastic scattering light from the surface of opaque sample, which is illuminated by a light sheet generated by a cylindrical lens. Through a simple structural modification by removing the fluorescent filter, the orthogonally imaging module can capture the elastic scattering image. As the opaque sample is scanned by a motorized stage, the light-sheet microscope acquires a serial of sectional images, which can be stitched to be a three-dimensional surface topography image. This method also offers the opportunity to visualize 3D fingerprint on micron level. Therefore, this technique may be useful in industry and biomedical field for the measurement of surface microstructure.
Citation
Fuhong Cai, Jie Chen, Chunling Zhou, Xuan Zhu, and Sailing He, "Exploratory Study on Light-Sheet Based Three-Dimensional Surface Topography," Progress In Electromagnetics Research, Vol. 161, 11-18, 2018.
doi:10.2528/PIER18012703
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