In this paper, the inversion method of mesospheric neutral wind is studied based on mid-latitude AgileDARN HF radar. Firstly, the meteor target observation method is carried out using 7.5 km range resolution and 2 s integration time. Then, the method of extracting the meteor echo from the data according to the doppler characteristics of the meteoris studied. Finally, the meridional and zonal components of mesospheric neutral wind are obtained by singular value decomposition method based on doppler velocity of meteor echo. The data analysis shows that the meteor echo has the highest incidence in the morning of local time and the lowest incidence in the evening of local time. The semi-diurnal characteristics of tidal waves can be seen from the meridional and zonal components of mesospheric neutral wind. Aiming at the ambiguity of elevation angle measured by AgileDARN HF radar, a method is proposed to reduce the ambiguity of elevation angle, and the wind field profile of mesospheric neutral wind along altitude is obtained, which lays a foundation for the subsequent study of gravity wave, tidal wave and planetary wave based on mesospheric wind field.