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Inhibition of Bone Formation by High Intensity Pulsed Electromagnetic Field in Mc3t3-E1 Cells

By Kangchu Li, Yanping Hui, Shirong Ma, Guirong Ding, Yao Guo, Junye Liu, Yurong Li, and Guozhen Guo
Progress In Electromagnetics Research, Vol. 112, 139-153, 2011


To investigate the effects of pulsed electromagnetic field (PEMF) with high electric field intensity on bone formation in murine osteoblast-like MC3T3-E1 cells, proliferation, alkaline phosphotase (ALP) activity, mineralized nodule formation, Collagen Type I (COL-I) and core-binding factor (Cbf)a1 mRNA expression, and bone morphogenetic protein (BMP)2/4 and mothers against decapentaplegic (Smad)1/5/8 protein expression were examined in cultured MC3T3-E1 cells after exposure to PEMF at the field intensities of 0kV/m, 50kV/m or 400kV/m for 400 consecutive pulses daily for 7 consecutive days. After 50 kV/m of PEMF exposure, none of the above parameters of MC3T3-E1 cells changed significantly when compared to the control groups. However, the proliferation, ALP activity and mineralized nodule formation of MC3T3-E1 cells in 400 kV/m PEMF exposure groups decreased significantly although COL-I and Cbfa1 mRNA expression and BMP2/4 and Smad1/5/8 protein expression did not change. The PEMF we used at high electric field intensity suppressed proliferation, differentiation and mineralization of MC3T3-E1 cells in culture and appeared to be harmful for bone formation.


Kangchu Li, Yanping Hui, Shirong Ma, Guirong Ding, Yao Guo, Junye Liu, Yurong Li, and Guozhen Guo, "Inhibition of Bone Formation by High Intensity Pulsed Electromagnetic Field in Mc3t3-E1 Cells," Progress In Electromagnetics Research, Vol. 112, 139-153, 2011.


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